After Jesus heals a demon-possessed man (Matthew 12:22), the Pharisees accuse Him of doing so by the power of Satan (Matthew 12:24; Mark 3:22). Jesus explains the illogic of their position and warns that blasphemy against the Holy Spirit is an unforgiveable sin (Matthew 12:31-32; Mark 3:29). Clearly, they had committed that unforgivable sin: a willful rejection of Jesus by saying that He was working by the power of Satan, not the Holy Spirit.
The leaders had regularly seen Jesus and personally witnessed His miracles, yet they still rejected Him. It is no wonder that Jesus began talking in parables to reveal truth to the receptive while preventing the leaders from understanding and being saved (Matthew 13:10–15).
Believers who worry that they've committed the unforgiveable sin likely have not. Those who willfully reject Jesus and besmirch the Holy Spirit by claiming that Christ's power is aligned with Satan wouldn't be sensitive enough to worry about their own sin.
Sometimes our worries are, themselves, proof that we have nothing to worry about. A parent who worries about being a good parent is likely a good parent because they care enough to worry. Someone who worries about having committed the unforgiveable sin likely hasn't because they care enough about Christ to have that concern. Besides, no one today can commit what Jesus declared the unforgiveable sin because none of us is in Christ's presence witnessing His miracles as the Pharisees were.
But our hearts can become so hard that they will no longer repent. This is because sin hardens hearts (Hebrews 3:13). If you come to know more about Christ and still reject him, your heart will harden (Hebrews 6:4–6). While there’s not a “tripwire” sin, you may lose the capacity or desire to repent.
This does not mean that God can’t overcome a hardened heart, nor does it mean that we should stop praying for those who have constantly rejected Jesus all their lives, but it does mean we need to watch ourselves not to assume there will be another chance to repent and be saved later.